脱细胞猪小肠粘膜下层基质材料中生长因子的定量检测

作者：本站编辑    发布日期：2021-03-10

Quantitative Detection of Growth Factors in Porcine Small Intestinal Submucosa Matrix Materials

 

作者：

张扬，夏磊磊，高建萍，门福民，郑晓龙，张晋辉，陈毅，赵博

中文摘要：

采取液氮冷冻粉碎技术将猪小肠粘膜下层脱细胞基质(SIS, Small Intestinal Submucosa)粉碎，再通过预处理将SIS材料中的三种活性生长因子包括碱性成纤维细胞生长因子(FGF2)、转化生长因子(TGFβ1)和血管内皮生长因子(VEGF)释放出来，以便采用特异性酶联免疫(ELISA)试剂盒检测，进而得到样品中三种活性生长因子的含量。两种不同的SIS产品(VIDASIS^TM和Biodesign^®)中的检测结果表明：VIDASIS^TM中TGFβ1的含量为8375±2125 pg/g、VEGF的含量为5486±1043 pg/g、FGF2的含量为3990±1372 pg/g；Biodesign^®中TGFβ1的的含量为11517±331 pg/g、VEGF的含量为5432±272 pg/g、FGF2的含量为5417±947 pg/g。

 

英文摘要：

In this study, three growth factors including basic fibroblast growth factor (FGF2), transforming growth factor β1 (TGFβ1) and vascular endothelial growth factor (VEGF) in two SIS products (VIDASIS^TM and Biodesign^®) were quantified by enzyme linked immune sorbent assay (ELISA) kit through the preliminary treatment of liquid nitrogen freezing and grinding technology which was aimed to release the growth factors from the tissue. Results showed that contents of TGFβ1, VEGF and FGF2 in VIDASISTM were 8375±2125 pg/g, 5486±1043 pg/g and 3990±1372 pg/g respectively; while the corresponding contents in Biodesign^® were 11517±1331 pg/g, 5432±272 pg/g and 5417±947 pg/g.

关键词：

猪小肠粘膜下层，脱细胞基质，转化生长因子，碱性成纤维细胞生长因子，血管内皮生长因子

Keywords：

Porcine Small Intestinal Submucosa, Acellular Matrix, Transforming Growth Factor, Basic Fibroblast Growth Factor, Vascular Endothelial Growth Factor

 

材料科学, 2018, 8(5), 595-602